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methocult gf 3534 medium  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc methocult gf 3534 medium
    Methocult Gf 3534 Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methocult gf 3534 medium/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    methocult gf 3534 medium - by Bioz Stars, 2026-03
    90/100 stars

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    Bone marrow colony-forming assays. Bone marrow cells (2 × 104) were cultured in <t>methylcellulose</t> media for 14 days. Each sample was plated in triplicate for wild-type (n = 8), 4E-BP1−/− (n = 8) and 4E-BP2−/− (n = 3) mice. (a) Colonies were counted. The total number of colonies per 20 000 cells (one plate) is shown. (b) Classification into CFU-granulocyte, -monocyte, and -granulocyte/monocyte (CFU-G, CFU-M and CFU-GM, respectively) according to standard morphological criteria. Results are expressed as percent total colony count.
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    Bone marrow colony-forming assays. Bone marrow cells (2 × 104) were cultured in methylcellulose media for 14 days. Each sample was plated in triplicate for wild-type (n = 8), 4E-BP1−/− (n = 8) and 4E-BP2−/− (n = 3) mice. (a) Colonies were counted. The total number of colonies per 20 000 cells (one plate) is shown. (b) Classification into CFU-granulocyte, -monocyte, and -granulocyte/monocyte (CFU-G, CFU-M and CFU-GM, respectively) according to standard morphological criteria. Results are expressed as percent total colony count.

    Journal: Immunology

    Article Title: Impaired myelopoiesis in mice lacking the repressors of translation initiation, 4E-BP1 and 4E-BP2

    doi: 10.1111/j.1365-2567.2008.02981.x

    Figure Lengend Snippet: Bone marrow colony-forming assays. Bone marrow cells (2 × 104) were cultured in methylcellulose media for 14 days. Each sample was plated in triplicate for wild-type (n = 8), 4E-BP1−/− (n = 8) and 4E-BP2−/− (n = 3) mice. (a) Colonies were counted. The total number of colonies per 20 000 cells (one plate) is shown. (b) Classification into CFU-granulocyte, -monocyte, and -granulocyte/monocyte (CFU-G, CFU-M and CFU-GM, respectively) according to standard morphological criteria. Results are expressed as percent total colony count.

    Article Snippet: Bone marrow cells (2 × 10 4 ) and spleen cells (1 × 10 5 ) were cultured at 37°, 5% CO 2 in a methylcellulose-based medium (MethoCult GF M 3534; Stem Cell Technology, Vancouver, BC, Canada) designed to support the formation of colonies derived from the myeloid lineage.

    Techniques: Cell Culture

    Spleen colony-forming assays. Spleen cells (1 × 105) were cultured in methylcellulose media for 14 days. Each sample was plated in triplicate for wild-type (n = 4), 4E-BP1−/− (n = 5) and 4E-BP2−/− (n = 3) mice. (a) Colonies were counted. The total number of colonies per 100 000 cells (one plate) is shown. (b) Classification into CFU-granulocyte, -monocyte, and -granulocyte/monocyte (CFU-G, CFU-M and CFU-GM, respectively) according to standard morphological criteria. Results are expressed as percentage of total colony count.

    Journal: Immunology

    Article Title: Impaired myelopoiesis in mice lacking the repressors of translation initiation, 4E-BP1 and 4E-BP2

    doi: 10.1111/j.1365-2567.2008.02981.x

    Figure Lengend Snippet: Spleen colony-forming assays. Spleen cells (1 × 105) were cultured in methylcellulose media for 14 days. Each sample was plated in triplicate for wild-type (n = 4), 4E-BP1−/− (n = 5) and 4E-BP2−/− (n = 3) mice. (a) Colonies were counted. The total number of colonies per 100 000 cells (one plate) is shown. (b) Classification into CFU-granulocyte, -monocyte, and -granulocyte/monocyte (CFU-G, CFU-M and CFU-GM, respectively) according to standard morphological criteria. Results are expressed as percentage of total colony count.

    Article Snippet: Bone marrow cells (2 × 10 4 ) and spleen cells (1 × 10 5 ) were cultured at 37°, 5% CO 2 in a methylcellulose-based medium (MethoCult GF M 3534; Stem Cell Technology, Vancouver, BC, Canada) designed to support the formation of colonies derived from the myeloid lineage.

    Techniques: Cell Culture